Coding

Part:BBa_K1634003:Design

Designed by: Xinhong Chen   Group: iGEM15_China_Tongji   (2015-09-13)


ChR2-YFP ( a kind of channelrhodopsin )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal XhoI site found at 286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 927
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1736
    Illegal SapI site found at 651


Design Notes

Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of ChR2-YFP, avoiding using the restriction site exits in it.


Source

ChR2 is a channelrhodopsin in green algae, but we didn't get it directly from the algae. Instead, we get ChR2 from some plamids by PCR.

References